the fold regulation of an individual gene 2−∆∆cq
Methods in molecular biology vol 1537 oral biology molecular techniques and applications 2nd edition
... Long ncRNA libraries Average number of RNA detected Average number of genes detected miRNA 386 RefSeq genes 3050 99 lncRNA genes 1419 piRNA Other ncRNAs 145 a Includes snoRNAs, tRNAs, snRNA, and ... with in situ hybridization (ISH) analysis to study gene expression in embryonic tissue explants The image shows the effects of BMP2 beads on ld1 gene expression in explants of calvarial mesenchyme ... substantially updated Epigenetics is now a major theme in biology and is providing great insight into how we interact with our environment As DNA methylation features heavily in epigenetic studies, new...
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Investigations on the roles of ubiquitin in the regulation of heat shock gene HSP70B 2
... Transcriptional Regulation 38 1.5.1 Introduction to Transcriptional Regulation in Eukaryotes 38 1.5.2 Mediator Complex in Transcriptional Regulation 39 1.5.3 The Role of Ubiquitin in Transcriptional Regulation ... specific for the named gene s mRNA h[protein name or gene name] Refers to a human protein or gene; used to distinguish between Saccharomyces cerevisiae and Homo Sapiens proteins or genes when both are ... Illustration 4: Hypothetical Model of HSP70B’ Regulation 171 List of Symbols None List of Conventions Gene name with only first letter capitalized, i.e Protein Hsf1 Gene name entirely capitalized, i.e...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
... in the regulation of transcription complexes More and more experiments have identified that proteins traditionally thought to be strictly cytoplasmic structural factors can influence gene regulation ... occur during morphogenesis to the nucleus, resulting in changes in gene expression via either the nuclear shuttling of 2678 Fig Model of Fil-I participation in transcription regulation Fli-I protein ... gamma-subunit gene cooperatively with the myogenin–E12 complex during myogenesis FEBS Journal 276 (2009) 2669–2685 ª 2009 The Authors Journal compilation ª 2009 FEBS 2679 Actin and ABPs in transcription regulation...
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báo cáo khoa học: " Characterization of Vitis vinifera NPR1 homologs involved in the regulation of Pathogenesis-Related gene expression" ppsx
... progress has been made in the model plant Arabidopsis thaliana in identifying genes that affect regulation of defense gene expression Several key plant defense regulators especially involved in ... characterized [4] The npr1 mutant was isolated in a genetic screen for plants that failed to express PR2 gene after SAR induction [5] NPR1 (Nonexpressor of PR genes 1) has been identified as a key positive ... carboxy-terminal leucinerich repeat (LRR) typical of resistance (R) genes [26] Besides putative R genes, the grape genome contains several candidate genes encoding putative signalling components for disease...
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NOVEL MECHANISMS IN THE REGULATION OF PERINUCLEAR ACTIN ASSEMBLY 2
... the nucleus and affect gene regulation This schematic, adapted from (Shivashankar, 2011), illustrates our current understanding of mechanosignaling to the nucleus and gene regulation, which at ... nuclear transport The nucleus contains most of the genetic materials of the cell and plays important roles as center of transcription and gene regulation Extracellular signals can be transduced ... noted that the Drosophila gene diaphanous, whose product was required for cytokinesis, shared two regions of sequence similarity with the murine ld gene and yeast cytokinesis genes Bni1p and Cdc12p...
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Investigations on the roles of ubiquitin in the regulation of heat shock gene HSP70B 1
... UBIQUITIN IN THE REGULATION OF HEAT SHOCK GENE HSP70B’ EE THIAM HEE, GARY NATIONAL UNIVERSITY OF SINGAPORE 2011 INVESTIGATIONS ON THE ROLES OF UBIQUITIN IN THE REGULATION OF HEAT SHOCK GENE HSP70B’...
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Investigations on the roles of ubiquitin in the regulation of heat shock gene HSP70B 3
... the Proteosome in Transcriptional Regulation 1.5.1 Introduction to Transcriptional Regulation in Eukaryotes A nucleated cell contains within its genome all of the genes necessary to adapt to a changing ... only which genes are expressed but also at what level This regulation of transcription is a complex and critical process in all cells which functions on various levels Aside from regulation stemming ... regulated This is not unusual as transcriptional regulation in general is the purview of a wide range of factors in complex symphony A Second Generation Timer Model has also been postulated to...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 2
... media, outgrowth colonies were generated from (a) umbilical cord blood mononuclear cells with typical cobblestone morphology Similarly, outgrowth cells could be generated from (b) fetal blood ... facilitate visualization Cobblestone colonies were generated from cord blood (a, b) In contrast, outgrowth colonies from fetal blood (c) were heterogeneous in nature, adopting morphologies ranging ... cells could be generated from (b) fetal blood mononuclear cells, which appear to have a more heterogeneous phenotype, adopting a more spindle morphology (c) When cultured in the same medium, fibroblastic...
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Tài liệu Characterization of the Polymorphic Behavior of an Organic Compound Using a Dynamic Thermal and X-ray Powder Diffraction Technique pptx
... solution was cooled to precipitate and isolate the solids as a wetcake In the following discussions, generalizations on the conditions found to produce the various crystalline forms of disodium salt ... representative samples of polymorph mixtures of are given in Table Forms I and II Figure Second generation Dow-developed DSC/XRPD instrument Disruption of the thermal environment of the DSC was ... cm length of the PSD subtended ∼25° 2θ of the diffraction pattern The DSC component was a second generation custom-built calorimetry cell, which had a temperature range of -45 to 600 °C, with temperature...
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Tài liệu Báo cáo khoa học: Understanding the binding properties of an unusual metal-binding protein ) a study of bacterial frataxin pdf
... Hum Mol Genet 11, 217–227 10 Delatycki MB, Williamson R & Forrest SM (2000) Freidreich’s ataxia: an overview J Med Genet 37, 1–8 11 Huynen MA, Snel B, Bork P & Gibson TJ (2001) The phylogenetic ... Koenig M (2000) Inactivation of the Friedreich ataxia mouse gene leads to early embryonic lethality without iron accumulation Hum Mol Genet 9, 1219–1226 Yoon T & Cowan JA (2003) Iron–sulfur cluster ... Hum Mol Genet 11, 2025–2036 Stehling O, Elsasser H-P, Bruckel B, Muhlenhoff U & Lill R (2004) Iron–sulfur protein maturation in human cells: evidence for a function of frataxin Hum Mol Genet 13,...
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Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt
... mimicking the proximal histidine of peroxidases and a COOH side chain of the antibody acting as a general acidbase catalyst like the distal histidine of peroxidases does EXPERIMENTAL PROCEDURES ... substituents in M KOH in 80% EtOH at room temperature [45] Production of monoclonal antibodies The generation of monoclonal antibodies has been reported in detail in previous papers [31,36,38] Fe(ToCPP) ... were then puri®ed from ascite ¯uid by protein A af®nity chromatography and their purity and homogeneity were checked by SDS gel electrophoresis Absorption spectroscopy measurements Absorption...
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Báo cáo khoa học: The catalytic mechanism of dye-decolorizing peroxidase DyP may require the swinging movement of an aspartic acid residue potx
... coupling steps) A great deal of information is available on the first step of the cycle, i.e the generation of compound I using H2O2 In the most popular model, a distal histidine is essential, ... of hydrogen peroxide stability of a novel Anabaena sp DyP-type peroxidase by site-directed mutagenesis of methionine residues Appl Microbiol Biotechnol 87, 1727–1736 16 Hofrichter M, Ullrich...
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Báo cáo khoa học: Regulation of Cyr61/CCN1 gene expression through RhoA GTPase and p38MAPK signaling pathways Role of CREB and AP-1 transcription factors doc
... of the GAPDH gene served as an internal control Regulation of Cyr61/CCN1 gene promoter According to TRANSFAC analysis [27], the promoter of either the human or mouse Cyr61/CCN1 gene (GenBank ... J.S (2001) Regulation of gene expression by the small GTPase Rho through the ERK6 (p38 gamma) MAP kinase pathway Genes Dev 15, 535–553 Treisman, R., Alberts, A.S & Sahai, E (1998) Regulation ... cells indicating that a relatively modest transcriptional regulation of the CTGF/CCN2 gene occurred as compared to that of the Cyr61/CCN1 gene Specificity of these hybridization signals was established...
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Báo cáo khoa học: Identification of domains involved in the allosteric regulation of cytosolic Arabidopsis thaliana NADP-malic enzymes ppt
... Regulation of A thaliana NADP-malic enzyme activity M C Gerrard Wheeler et al require further effort, as the gene family of this protein is more complex ... is more complex than expected [4] The Arabidopsis thaliana genome contains four NADP-ME genes [3,4] One gene encodes a plastidic enzyme (NADP-ME4 [3]), but the other three isoenzymes not possess ... structural and kinetic properties [3,5] Specifically, the isoenzymes behave differently in terms of regulation by metabolic effectors, NADP-ME2 being the most highly regulated, especially by activation...
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Báo cáo khoa học: The role of residues R97 and Y331 in modulating the pH optimum of an insect b-glycosidase of family 1 pdf
... reagents, unless otherwise specied, were purchased from Sigma or Merck Site-directed mutagenesis Site-directed mutagenesis was performed using as template the plasmid pT7-7 [15] containing as insert ... experiments were carried out following the instructions of the QuikChange site-directed mutagenesis kit (Stratagene) Primers used were: R97K, 5Â-GCCTGGACGCTTACA AGTTCTCCCTCTCCTG-3Â and 5Â-CAGGAGAGGGA ... ll these gaps, residues Y331, R97 and E187 of Sfbgly50 were replaced through site-directed mutagenesis by phenylalanine (Y331F), methionine (R97M), lysine (R97K) and aspartate (E187D) The effect...
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hack the stack - using snort & ethereal to master the 8 layers of an insecure network
... breach the network or, even worse, gain physical access to a server or piece of equipment It’s a generally accepted fact that if someone gains physical access to an item, they can control it.The ... CHAP uses a predefined secret and a pseudo random value that is used only once (i.e., a hash is generated and transmitted from client to server).This facilitates security because the value is...
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Báo cáo khoa học: The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines pdf
... site-specific mutagenesis introducing the K169E substitution in mammalian expression constructs and in GST fusion proteins Sitedirected mutagenesis was performed with the Quick-Change Mutagenesis kit ... phenotypes in vivo Nat Genet 33, 396–400 31 Alarcon-Vargas D & Ronai Z (2002) p53-Mdm2 – the affair that never ends Carcinogenesis 23, 541–547 32 Woods DB & Vousden KH (2001) Regulation of p53 function ... stabilized by VRK2B was acetylated de novo Discussion Alternative splicing of the human VRK2 gene generates two isoforms with a common kinase domain and a different C-terminus, which determines...
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